human epidermoid squamous carcinoma a431 cell lines  (ATCC)

Journal: Journal of Clinical Medicine

Article Title: An Evaluation of Prognostic Factors in Cutaneous Squamous Cell Carcinoma: A Single-Center Study of 237 Japanese Cases

doi: 10.3390/jcm14041243

Figure Lengend Snippet: List of top 10 differentially expressed genes (DEG) in A431 stimulated with LL-37.

Article Snippet: Human epidermoid squamous carcinoma A431 cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Efficient inhibition of EGFR signalling and of tumour growth by antagonistic anti-EGFR Nanobodies

doi: 10.1007/s00262-006-0180-4

Figure Lengend Snippet: Trivalent, bispecific Nanobodies inhibit A431 tumour cell growth in vitro and growth of A431 xenografts in athymic mice. a–c A431 epidermoid carcinoma cells were grown in serum-free medium supplemented with insulin, transferrin and selenium. After 24 h, medium was refreshed and dilutions (100, 10, 1 and 0 nM) of trivalent Nanobodies (a Ia1; b IIIa3; c L2–3.40) were added, together with 1 ng/ml (approximately 170 pM) of human EGF. After 2 days of culture, cells were pulsed with [3H]- thymidine and assayed 24 h later for the incorporation of radioactivity in genomic DNA. d Groups of 8 mice were subcutaneously injected with A431 cells (107 cells per mouse). One day later, treatment was started by intra-peritoneal injection of 1 mg of purified trimeric Nanobody. Mice were treated twice weekly with the same quantity of Nanobody for up to 4 weeks (indicated by arrows). Relative tumour volume (RTV) was measured as function of time. Squares solvent control (PBS); triangles IaI; inverse triangles IIIa3 and diamonds L2–3.40. Error bars indicate the standard error of the mean of 8 mice

Article Snippet: Human epidermoid squamous carcinoma cell line A431 [ 19 ], carrying an amplification of the EGFR gene [ 36 ], was obtained from the ATCC (cat. nr. CRL-1555).

Techniques: In Vitro, Radioactivity, Injection, Purification, Solvent, Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Efficient inhibition of EGFR signalling and of tumour growth by antagonistic anti-EGFR Nanobodies

doi: 10.1007/s00262-006-0180-4

Figure Lengend Snippet: Selected Nanobodies specifically recognise the EGFR. a The binding of anti-EGFR Nanobody Ia1 displayed on phage and of control phage (not expressing any antibody fragment) to EGFR-negative cell line 3T3 2.2 and EGFR-expressing cell line Her14 was determined by cell-ELISA. Error bars indicate the standard deviation of three independent results. b FACS staining of cell lines expressing EGFR (A431 and Her14) and of the EGFR-negative cell line 3T3 2.2 with FITC-coupled anti-EGFR Nanobody Ia1 (black line) or control Nanobody (grey fill). c Immuno-precipitation (IP) of EGFR from Hela cell lysates using biotinylated anti-EGFR Nanobody Ia1. Lanes 1–3 total cell lysates used for IP. Lanes 4–6 IP with streptavidin beads only, with an anti-glutathion S transferase (control) Nanobody and with the anti-EGFR Nanobody Ia1, respectively. Blots were stained for EGFR as described in Materials and methods

Article Snippet: Human epidermoid squamous carcinoma cell line A431 [ 19 ], carrying an amplification of the EGFR gene [ 36 ], was obtained from the ATCC (cat. nr. CRL-1555).

Techniques: Binding Assay, Control, Expressing, Enzyme-linked Immunosorbent Assay, Standard Deviation, Staining, Immunoprecipitation

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Efficient inhibition of EGFR signalling and of tumour growth by antagonistic anti-EGFR Nanobodies

doi: 10.1007/s00262-006-0180-4

Figure Lengend Snippet: Immunisation of Llama glama with EGFR-containing cell preparations induces a strong anti-EGFR humoral immune response. The reactivity of pre-immune (day 0: diamonds) and immune sera (day 28: squares and day 42: triangles) of animals immunised with whole, intact A431 cells (a, b, e), or with A431-derived membrane vesicles (c, d) towards A431 cells (a, c), towards EGFR-negative 3T3 2.2 cells (b, d) and towards purified EGF receptor (e) was determined by whole cell ELISA (a–d) or conventional ELISA (e) as described in Materials and methods

Article Snippet: Human epidermoid squamous carcinoma cell line A431 [ 19 ], carrying an amplification of the EGFR gene [ 36 ], was obtained from the ATCC (cat. nr. CRL-1555).

Techniques: Derivative Assay, Membrane, Purification, Enzyme-linked Immunosorbent Assay